Jin and Direct Electron, LP (San Diego, CA) for support and access to their demonstration DE. For a lytic phage, growth is primarily determined via effects on any of three properties ( Adams, 1959 ): the rate the phage infects cells (adsorption rate), the number of progeny per infection (burst), and the time from infection until progeny are released (lysis time or latent period). Vimr for the kind gift of phage CUS-3 and its host E. Phage Capsule Depolymerases against K1, K5, and K30 Capsulated E. Johnson (Scripps Research Institute) for helpful discussions, and E. Molineux Published 1 March 2010 Biology Virology. The phage huntwhich included combing through pre-existing phage libraries (i.e. Pogliano (UCSD) for access to equipment and laboratory space, R. Navy, to find phages that could kill Pattersons A. Throughout, we explore how the modern scientific community works to improve the reliability and success of phage therapy in the clinic and discuss how to properly evaluate the potential for phage therapy to combat antibiotic-resistant bacteria.Ĭopyright © 2019 Elsevier Inc. The team relied on researchers from the Center for Phage Technology at Texas A&M University, as well as scientists from the U.S. We also examine the use of animal models, case studies, and results from clinical trials. Molineux Prior studies treating mice infected with Escherichia coli O18:K1:H7 observed that phages requiring the K1 capsule for infection (K1-dep) were superior to. We compare and contrast phage therapy and chemical antibiotics, highlighting their potential synergies when used in combination. Here, we review the history of phage therapy research. The system is based upon a set of vectors that carry the origin of replication of the broad host range mobilizable plasmid RSF1010. Nevertheless, bacteria can readily evolve phage resistance too, making it crucial for modern phage therapy to develop strategies to capitalize on this inevitability. Here we describe the development of a genetic system that utilizes the transferral of extrachromosomally-replicating DNA by conjugation from an Escherichia coli donor strain into Haemophilus recipient strains. There also have been recent case reports of phage therapy demonstrating clinical utility in resolving these otherwise intractable infections. This stems from the rise in frequency of multi-drug-resistant bacterial infections in humans. Phage therapy, long overshadowed by chemical antibiotics, is garnering renewed interest in Western medicine.
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